Dengue NS1 Ag ELISA kit
INTENDED USE
The Dengue NS1 Ag ELISA Kit is a solid-phase enzyme-linked immunosorbent assay for the qualitative detection of dengue
NS1 antigen (DEN1, 2, 3, 4) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of an
acute infection with dengue viruses.
| Product details |
Description |
| Delivery |
Within 48 hours |
| Packaging Specifications |
8 x 12 strips, 96 wells |
| Country Of Origin |
China |
| Manufacturer |
18 months |
| Preservation method |
2℃-8℃ |
| Specimen |
Whole blood |
| Assification |
class1 |
| Type |
Elisa Test Kit |
PRINCIPLE
The Dengue NS1 Ag ELISA Kit is a solid phase enzyme linked immunosorbent assay (ELISA) based on the “Direct Sandwich”
principle. The microwells are coated with Anti-dengue NS1 antibodies with high reactivity for Dengue NS1 Ag. The samples
are added in the wells followed by addition of enzyme conjugate (monoclonal anti-dengue NS1 antibodies linked to Horse
radish Peroxidase(HRP). A sandwich complex is formed in the well wherein dengue NS1 (from serum sample) is“trapped” or
“sandwiched” between the antibody and antibody HRP conjugate. Unbound conjugate is then washed off with wash buffer.
Upon addition of the substrate buffer and chromogen, a blue colour develops. To limit the enzyme-substrate reaction, stop
solution is added and a yellow colour develops which isfinally read at 450nm spectrophotometrically
SUMMARY
Dengue virus is a flavivirus found largely in areas of the tropic and sub-tropics. There are four distinct but antigenically
related serotypes of dengue viruses, and transmission is by mosquito,prinicipally Aedes aegypti and Aedes albopictus.
The mosquito-borne dengue viruses (serotype 1-4) cause dengue fever, a severe flu-like illness.The disease is prevalent in
third world tropical regions and spreading to sub-tropical developed countries - including the United States. WHO estimates
that 50-80 million cases of dengue fever occur worldwide each year, including a potentially deadly form of the disease called
dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS). Primary infection with dengue virus results in a self
limiting disease characterized by mild to high fever lasting 3 to 7 days, severe headache with pain behind the eyes, muscle
and joint pain, rash and vomiting. Secondry infection is the more common form of the disease in many parts of Southeast
Asia and South America. Thisform of the disease is more serious and can result in DHF and DSS. The major clinical symptoms
can include high fever, haemorrhagic evets, and circulatory failure, and the fatality rate can be as high as 40%. Early
diagnosis of DSS is particularly important, as patients may die within 12 to 24 hours if appropriate treatment is not
administered.
Primary dengue virus infection is characterized by elevations in specific NS1 antigen levels 0 to 9 days after the onset of
symptoms; this generally persists upto 15 days. Earlier diagnosis of Dengue reduces risk of complication such as DHF or DSS,
especially in countries where dengue is endemic.
Storage
• Store at 2-8℃.
• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date whichever is earlier.
• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at-20 °C. Avoid multiple freeze-thaw cycles.
• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.
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